Fig. 13.

Effects of DIDS on fluid secretion rates of isolated Aedes Malpighian tubules. A: effect of 200 μM DIDS in unstimulated Malpighian tubules. Lines connect control (unstimulated) and experimental (DIDS) data (filled circles) of each tubule for paired comparisons. Transepithelial fluid secretion rates were measured in the absence and presence of DIDS for 30 min each. Open circles indicate mean (±SE) secretion rates. The number of paired tubule measurements is shown in parenthesis. B: effect of 200 μM DIDS on the diuresis stimulated by aedeskinin III (AKIII; 10⁻⁶ M). Each Malpighian tubule was first studied for 30 min under unstimulated conditions, then in the presence of AKIII, and finally in the presence of AKIII and DIDS. C: effect of 200 μM DIDS on the diuresis stimulated by Anopheles calcitonin-like peptide (CLP; 10⁻⁶ M) following the experimental protocol in B. ^a,bP < 0.01, categorization of the means as determined by a repeated-measures ANOVA and Newman-Keuls posttest.