Fig. 7.
Expression of AeAE immunoreactivity in female Aedes Malpighian tubules. A: representative Western blots of total membrane protein (30 μg/lane) isolated from Malpighian tubules of adult Aedes females. Labeled arrows indicate the protein bands (a–e) displaying AeAE immunoreactivity. The antibodies used are indicated. Migrations of the molecular mass markers (in kDa) are indicated at left. B: representative Western blot of anti-AENt immunoreactivity in crude Malpighian tubule lysates (30 μg protein/lane) denatured under standard Laemmli (SDS) or high-urea conditions.