Fig. 9.

Localization of AeAE immunoreactivity in consecutive sections of female Aedes Malpighian tubules. Representative immunoperoxidase labeling of AeAE is shown in isolated Malpighian tubules from adult Aedes females. The antibodies used are indicated. The sections in A and B are from the same Malpighian tubule, taken 4 μm apart, and provide transverse sections through the armlike projections of stellate cells; the sections in C and D are as described in A and B but provide longitudinal sections through the armlike projections of a stellate cell. The arrows indicate red staining associated with the immunolabeling. The blue counterstain (hematoxylin) labels nuclei and provides contrast. N, the nucleus of a principal cell; n, the nucleus of a stellate cell.