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Effect of serum deprivation on GM-CSF expression by AEC. Primary murine AEC were placed in culture as described in materials and methods. On day 3 the medium was changed to control medium (10% serum) or medium without serum (0% serum). The cells were maintained in normoxia throughout. GM-CSF protein (A) and steady-state mRNA (B) were measured after 48 h. Data are expressed as means ± SD, n = 3. *P < 0.0002.
