Fig. 1.
SHP2 inhibition causes endothelial barrier dysfunction. A: changes in resistance across monolayers were measured after transient transfection of equivalent numbers of pulmonary artery endothelial cells (PAEC) with GFP (dashed line) or SHP2C459S (solid line) for 48 h. Data are presented as means ± SE of the resistance across the SHP2C459S monolayers normalized to the resistance across the GFP-transfected monolayers. Means ± SE; n = 3; *P < 0.0001 vs. GFP. A representative immunoblot probing for SHP2 is shown. B: changes in resistance across PAEC monolayers were measured following exposure to vehicle or NSC-87877. Arrow indicates addition of treatment. Means ± SE; n = 4; *P < 0.05 vs. vehicle for all doses.