Fig. 2.

Overexpression of catalytically inactive SHP2 promotes stress fiber formation and adherens junction disassembly. Equivalent numbers of PAEC were transfected with GFP or SHP2^C459S for 48 h. Endothelial cells were fixed and immunofluorescently stained for filamentous actin with phalloidin and for β-catenin or VE-cadherin with protein-directed antibodies. Insets are the same endothelial cells viewed for overexpression of GFP (left) or overexpression of SHP2^C459S by costaining with anti-HA antibody (right). Arrows indicate intercellular gapping; arrowheads denote increased stress fiber formation. Scale bars, 20 μM; n = 3.