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. 2010 Jan 29;11(3):208–213. doi: 10.1038/embor.2009.279

Figure 4.

Figure 4

ER antagonist ICI 182,780 reduced E2-dependent induction of DNA-PKcs. (A) MELN cells were grown in phenol red-free medium supplemented with 10% charcoal–dextran-stripped FBS for 3 days. After pretreatment with 1 μM of ICI 182,780 and 100 nM of E2 for 20 h, cells were treated with IR (10 Gy) and incubated for 2 h. Cell lysates were assayed for expression of proteins with the indicated antibodies. (B) MELN cells were grown as above. After ±pretreatment with 10 μM of CGK733 and 100 nM of E2 for 20 h, cells were exposed to IR (10 Gy) and incubated for 2 h. Cell lysates were assayed for expression of proteins with the indicated antibodies. Chk2, checkpoint kinase 2; DNA-PKcs, DNA-dependent protein kinase catalytic subunit; E2, oestrogen; ERα, oestrogen receptor-α; FBS, fetal bovine serum; IB, immunoblotting; IR, ionizing radiation.