Figure 2.

Clustering, gene ontology and binding-site analysis of putative JAK/STAT target genes. (A) Hierarchical cluster analysis of 1,168 Upd-regulated genes using log₂ ratios. The blue tiles represent downregulated genes and red tiles indicate upregulated genes. Six main clusters were identified. (B) The functional enrichment of the 1,168 Upd-regulated genes according to gene ontology terms. (C) The average number of potential 3n and 4n STAT92E-binding sites present in the upstream region of positively regulated genes at each time point, compared with the number of sites in a randomly selected ‘control' group of genes. ^*P<0.05, ^**P<0.005, ^***P<0.0005. JAK, Janus kinase; STAT, signal transducer and activator of transcription; Upd, Unpaired.