Figure 11.

Western blot analysis of retinal outer segment membrane protein 1 (Rom1), Prph2, and Rho in retinas from 3-week-old Rom1^+/+, Rom1^Rgsc1156/+, and Rom1^{Rgsc1156/Rgsc1156} mice (n=6 each). A: Western blots of 10 µg of retinal protein with antibodies to Rom1, Prph2, and β-actin. The stripped membrane was reprobed with antibodies to Prph2 and β-actin. B: Western blots of 0.1 µg of retinal protein with antibodies to Rho and β-actin. The stripped membrane was reprobed with β-actin antibodies. C: Semiquantitative analysis of Rom1, Prph2, and Rho levels. The western blot band intensities were measured with ImageJ software. Rom1, Prph2, and Rho protein levels were normalized to β-actin levels. The intensity (mean±standard error) was normalized to wild-type values, which were set equal to 100%. Protein levels differed significantly among the genotypes (* indicates p<0.05, Tukey–Kramer multiple-comparisons test).