Figure 4.

Luciferase activities for detection of protein–protein interactions involving various βA3-crystallin deamidation mutants. Luciferase activity values are expressed as fold activation relative to the basal control (pCMV-AD+pCMV-BD). Various plasmid constructs were co-transfected as labeled. Data represent the mean±SEM of results in three independent experiments. Group differences were all compared with wild type homodimer interaction (pAD-βA3+pBD-βA3). The asterisk indicates a p<0.05 and the double asterisk indicates a p<0.01.