Table 1. Primers for sub-cloning of βA3 genes and site-directed mutagenesis.
| Constructs | Forward primer | Reverse primer |
|---|---|---|
|
Sub-cloning | ||
| βA3-crystallin |
TTGGATCCGAGACCCAGGCTGAG |
GCTAAGCTTCTACTGTTGGATTCG |
|
Mutagenesis | ||
| βA3ΔG91 |
GTTTATCCTGGAGAGAGAATACCCTCGCTG |
CAAATAGGACCTCTCTCTTATGGGAGCGAC |
| βA3Q85E |
CTGTGGGCAAGAGTTTATCCTGG |
GACACCCGTTCTCAAATAGGACC |
| βA3Q180E | CGTGGGTATGAGTATATCTTGG | GCACCCATACTCATATAGAACC |
The underlined sequences are BamHI and HindIII restriction enzyme sites for forward and reverse primers, respectively.