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. 2010 Mar 16;5(3):e9667. doi: 10.1371/journal.pone.0009667

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Pereira-Rodrigues et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Fluorescent micrographs of HepG2 cells cultured on PS, ParC/PDMS, or PDMS substrata after staining with Calcein-AM (green) and Hoechst (blue) fluorescent dyes on Day 2, 8, or 14. Scale bar = 200 µm. B) Cell topography of HepG2 at Day 2 cultured on PS, ParC/PDMS, or PDMS substrata recorded using FeCN₆ ^4- oxidation potential for SECM ( = 5 mmol/L, E_applied = 0.5 V vs. Ag/AgCl). Scan area = 400×400 µm.

Inline graphic — A) Fluorescent micrographs of HepG2 cells cultured on PS, ParC/PDMS, or PDMS substrata after staining with Calcein-AM (green) and Hoechst (blue) fluorescent dyes on Day 2, 8, or 14. Scale bar = 200 µm. B) Cell topography of HepG2 at Day 2 cultured on PS, ParC/PDMS, or PDMS substrata recorded using FeCN₆ ^4- oxidation potential for SECM ( = 5 mmol/L, E_applied = 0.5 V vs. Ag/AgCl). Scan area = 400×400 µm.