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. 2010 Mar 16;5(3):e9724. doi: 10.1371/journal.pone.0009724

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Nijland et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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P_spac = IPTG inducible promoter, P_divIVA = constitutive B. subtilis divIVA promoter used to drive expression of lacZ, AmpR = ampicillin resistance gene, SpcR = spectinomycin resistance gene. Plasmid pLK has been made available through the Bacillus Genetic Stock Centre, with accession number ECE219.