Table 1. Transformation efficiency of tested strains using LB culture medium.
Strain | Transformants/106 cellsa at [IPTG] | ||
0 µM | 200 µM | 1000 µM | |
168 wt | 0.6 | - | - |
168-pLK | 9.8 | 16.3 | 8.7 |
NCIB3610-pLK | <0.01 | - | 2.8 |
JW49-pLK | <0.01 | - | 0.35 |
At the time of plating, the 168 and 168-pLK culture contained 1.3*109 viable cells/ml, the NCIB3610-pLK culture contained 0.9*109 viable cells/ml, and the JW49 contained 1.0*109 viable cells/ml. For transformations 6 µg chromosomal DNA from strain JWV184 per ml culture was used. - = not tested.