Figure 4.

Heat shock sensitivity of a wsc1wsc2wsc3Δ mutant. Cells were grown on YPD containing 1 M sorbitol at 30°C until they reached an OD₆₀₀ of 1. Cultures were washed and resuspended in SC medium. They were shifted to 50°C for the indicated periods of time or first shifted to 37°C for 30 min followed by the shift to 50°C. After heat shock, the cells were plated on YPD containing 1 M sorbitol and scored for growth after 2–3 days at 30°C. The results are shown as percent survival relative to the cultures before treatment at 50°C. The strains are SP1, wild type, or the ALH758 wscΔ strain. Results are from three experiments.