Figure 5.

Suppression of the heat shock sensitivity of the wsc1wsc2wsc3Δ strain by overexpression of IRA2 or by deletion of RAS2. (A) The wsc1wsc2wsc3Δ (ALH758) strain was transformed with IRA2 or a control plasmid. Transformants were grown for 2 days and were then shifted to 50°C for the indicated times. Cells were diluted, plated, and grown as in Fig. 4. The wild-type strain (SP1) was used for comparison. (B) The wild type (SP1), wsc1wsc2wsc3Δ (ALH758), and wsc1wsc2wsc3ras2Δ (JV758Δras) strains were grown and treated as in Fig. 4.