Table 1.
Phenotypes caused by deletion of WSC
| Genotype | Growth on YPD 28°C | Growth on YPD 37°C | Growth on SC 28°C | Growth on SC 37°C | Caffeine sensitivity SC 28°C | Glycogen accumulation | Staurosporine sensitivity SC 28°C | Nitrogen starvation sensitivity |
|---|---|---|---|---|---|---|---|---|
| Wild type | + | + | + | + | − | + | − | − |
| wsc1Δ | + | − | + | + | − | + | − | − |
| wsc1wsc3Δ | + | − | + | − | + | − | ± | − |
| wsc1wsc2Δ | − | − | + | − | + | − | ± | − |
| wsc1wsc2wsc3Δ | − | − | + | − | + | − | + | ± |
YPD, 1% yeast extract, 2% peptone, 2% dextrose; SC, synthetic complete medium containing yeast nitrogen base at 0.67 g/liter, 2% dextrose, and amino acid supplements. Cells were streaked on plates to test for growth at the designated temperatures, sensitivity to caffeine (3 mM), and staurosporine (1 μg/ml). Plates were scored after 2 days. Glycogen accumulation was determined by iodine staining of patches on SC plates. Nitrogen starvation was tested after 2 weeks of incubation on plates lacking a nitrogen source.