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Suppression of the heat shock sensitivity of mutants in the RAS–cAMP pathway
| CDC25 | |||
|---|---|---|---|
| GDP-RAS ⇄ GTP-RAS → CYR1/CAP → cAMP → BCY1/ | |||
| IRA1/IRA2TPK1, TPK2, TPK3 | |||
|
| |||
| Mutant strains | Genotype | WSC1 | WSC2 |
| IR2.53 | ira1Δira2ΔR2Y64 | + | + |
| JF36A | ras1Δras2ΔpCYR1 | + | − |
| TF1.5prC | bcy1Δtpk2Δtpk3Δtpk1w | − | − |
Cells were transformed with high-copy-number/plasmids expressing WSC1 or WSC2 and tested for heat shock sensitivity at 55°C. +, Growth; −, no growth after heat shock treatment.
