Table 3.
Percentages of patients showing positive immunohistochemical reactions to p-ELK-1, ATF-2 [31], p50 and p65 [5] in normal prostate (NP), benign prostatic hyperplasia (BPH) and prostatic carcinoma (PC) and average optical densities of immunostaining in positive patients.
| p-ELK-1 | p-ATF-2 | p50 | p65 | |||||
|---|---|---|---|---|---|---|---|---|
| Prostates (no.) | Positive cases (%) | Optical density | Positive cases (%) | Optical density | Positive cases (%) | Optical density | Positive cases (%) | Optical density |
| Normal (20) | 100 | 2.41 ± 0.9a | 100 | 3.34 ± 0.83a | 60 | 7.88 ± 2.4* | 0 | |
| BPH (35) | ||||||||
| Cytoplasm | 14.28 | 9.42 ± 5.13* | 100 | 18.6 ± 1.72# | 71.4 | 9.08 ± 3.23* | ||
| Nucleus | 82.85 | 10.52 ± 2.56b | 45.71 | 5.25 ± 2.16a | ||||
| PIN | ||||||||
| Low grade PIN (12) | 100 | 3.22 ± 0.7a | 100 | 4.32 ± 1.09* | 58.3 | 15.32 ± 2.00# | 0 | |
| High grade PIN (15) | ||||||||
| Cytoplasm | 60 | 17.35 ± 1.45# | 53.3 | 13.35 ± 1.78# | 86.6 | 27.51 ± 2.79§ | 93.3 | 30.17 ± 3.08# |
| Nucleus | 40 | 18.27 ± 2.31c | 26.6 | 12.31 ± 4.21b | 26.6 | 28.76 ± 3.31a | 13.3 | 29.77 ± 2.13a |
| PC | ||||||||
| Low Gleason (21) | ||||||||
| Cytoplasm | 66.6 | 19.62 ± 5.64# | 61.9 | 12.68 ± 3.28# | 80.9 | 28.01 ± 2.25§ | 100 | 23.8 ± 2.93§ |
| Nucleus | 42.85 | 18.65 ± 3.4c | 23.8 | 10.58 ± 1.2b | 30.1 | 32.08 ± 2.96a | 19 | 28.34 ± 2.04a |
| Medium Gleason (51) | ||||||||
| Cytoplasm | 72.54 | 17.75 ± 4.54# | 68.62 | 11.75 ± 3.45# | 94.1 | 29.17 ± 1.22§ | 92.1 | 29.09 ± 1.90# |
| Nucleus | 37.25 | 17.23 ± 3.56c | 19.6 | 9.56 ± 3.87b | 41.2 | 33.82 ± 4.07a | 45.1 | 34.91 ± 4.32b |
| High Gleason (21) | ||||||||
| Cytoplasm | 85.71 | 19.76 ± 4.78# | 76.19 | 12.98 ± 5.11# | 100 | 39.37 ± 4.15¥ | 80.9 | 36.71 ± 2.18¥ |
| Nucleus | 33.3 | 18.76 ± 3.87c | 14.28 | 13.43 ± 3.56b | 47.6 | 41.26 ± 2.31b | 71.4 | 46.56 ± 1.20c |
Average optical densities were evaluated only in patients showing positive immunoreactions. Statistical analysis refers to each antibody separately. Statistical analysis also separated cytoplasmic (symbol superscripts) and nuclear (letter superscripts) values. Values denoted by different superscripts are significantly different from each other. Significance was determined by the one way ANOVA test at p ≤ 0.05, by multiple pairwise comparisons (GraphPad PRISMA 3.0 computer program).