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Real Time PCR: Significantly increasing gene expression of IL-10, TGF-β, TNF-α but a decrease of IFN-γ at metastatic sites in the liver on day 10, 15 and 20 after intraportal injection of 1 × 105 CT26.WT cells. Analysis was measured to control tissue (liver). Lines with asterisks show significant differences during metastatic growth.
