Table 2.
LAMP and PCR primers used in this study to detect Vibrio parahaemolyticus
| Primer name | Sequence (5'-3') | Positiona | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| F3 | TTGGATTCCACGCGTTAT | 528-545 | Ladder-like bands for LAMP; 183 bp for F3/B3 PCR | This study |
| B3 | CGTTCAATGCACTGCTCA | 693-710 | ||
| FIP | TGAGATTCCGCAGGGTTTGTAA TTATTTTTGGCACTATTACTACCG |
587-608 (F1c) 547-570 (F2) |
||
| BIP | GTTCCGTCAGATTGGTGAGTATC TAGAAGGCAACCAGTTGTT |
609-631(B1c) 673-691(B2) |
||
| Loop | AGAACGTACCAGTGATGACACC | 632-653 | ||
| toxR-F | GTCTTCTGACGCAATCGTTG | 453-472b | 367b | [18] |
| toxR-R | ATACGAGTGGTTGCTGTCATG | 799-819b |
a The positions are numbered based on the coding sequence of V. parahaemolyticus strain AQ3815 toxR gene [GenBank: L11929].
b Differences in primer positions and amplicon size were noted from those originally published after reanalysis of the sequences.