Figure 2.

The growth curves of L. pneumophila wild-type JR32, the LpΔclpP mutant, both harboring the vector pBC(gfp)Pmip, and the complemented strain LpΔclpP-pclpP. Overnight cultures of mid-exponential bacterial cells were diluted into fresh medium and then incubated at (A) 25°C, (B) 30°C, (C) 37°C, and (D) 42°C, respectively. Growth was monitored by OD₆₀₀ at various time points. Points indicate mean values and error bars indicate standard deviations of three experiments.