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Device response to unit changes in pH. For time < 0, a pH 8.0 buffer was present in the reservoir. At time = 0 (red arrow) this buffer was exchanged with a buffer at pH 9.0 and at time = 57.25 s (green arrow) a second exchange, with a pH 10.0 buffer, was performed. All buffers were 0.01X PBS with 150 mM NaCl and were titrated using NaOH and HCl.
