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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cell Death Differ. 2009 Oct 23;17(4):699–709. doi: 10.1038/cdd.2009.156

Figure 1. IRF-1 induces apoptosis in gastric cancer cells.

Figure 1

(a) Cells were infected with Ad-IRF-1, the empty vector Ad-ψ5 (Ad-Null) or media only (no infection, NI) at 50 MOI for the indicated times. Apoptosis was detected by FACS analyses of Annexin V-FITC staining and propidium iodide (PI) staining as described in Materials and Methods, with representative bivariate data shown. (b) Samples including above were analysed in three independently treated experiments (N=3) and the mean percent apoptosis +/− standard deviation is indicated graphically. (c)–(h) AGS cells were either treated with indicated amount of Ad-IRF-1 for 24 h, or treated with 50 MOI for the indicated times; and tetracycline inducible IRF-1 stably-transfected AGS cells (AGSI) were treated with 2 µg/ml tetracycline at indicated times. Whole-cell lysates were harvested and cell extracts (20 µg) were immunoblotted for IRF-1, PARP (c,d,e), caspase-8, caspase-3 and caspase-9 (f,g,h). β-actin was used as an internal loading control.