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. Author manuscript; available in PMC: 2011 Mar 12.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Feb 10;393(3):471–475. doi: 10.1016/j.bbrc.2010.02.025

Fig. 1.

Fig. 1

DMT1 does not mediate 45Ca2+ transport in Xenopus oocytes. (A) Radiotracer metal-ion (*Me2+) uptake (pH 5.5, 1 mM Mg2+) in control oocytes and oocytes expressing human DMT1 (n = 9-13). We compared uptake of 2 μM 55Fe2+ with that of 2 μM 45Ca2+. Two-way ANOVA revealed an interaction (P < 0.001); a within 45Ca2+ uptake, DMT1 did not differ from control (unadjusted P = 0.933). (B) Uptake of 100 μM 45Ca2+ (pH 5.5) in the absence (None) or presence (+Fe2+) of 100 μM Fe2+ in control oocytes and oocytes expressing human DMT1 (n = 22-32). (Note the y-axis scale is one tenth that in A.) Two-way ANOVA revealed a main effect of DMT1 (P < 0.001) but not of [Fe2+] (P = 0.99), and no interaction (P = 0.094).