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Values for the N−β5 are derived from kinetic data for UbL50E and pWTUb while values for the N−310, −β5 are obtained from NSHX studies on UbL50E intermediate. The energy of the UbL50E at pH 7.8 with the β5 strand folded is obtained from the apparent pKa of the CD-monitored pH-induced unfolding transition (pH 5.8, see Fig. 2).
