. Author manuscript; available in PMC: 2011 Apr 2.

Published in final edited form as: J Mol Biol. 2010 Feb 6;397(3):777–788. doi: 10.1016/j.jmb.2010.01.056

Table 1.

Kinetic parameters obtained from chevron analysis¹

Protein	Solvent condition	m_f (kcal mol⁻¹ M⁻¹)	m_u (kcal mol⁻¹ M⁻¹)	ΔG_f^‡(0) (kcal mol⁻¹)	ΔG^o(kcal mol⁻¹)
pWTUb (Fig. 2)	N-state pH 7.8	0.60 ± 0.04	0.35 ± 0.02	3.97 ± 0.24	−8.64 ± 0.35
pWTUb (Fig. 2)	N-state pH 4.0	0.64 ± 0.03	0.36 ± 0.02	4.62 ± 0.21	−9.18 ± 0.28
L50E (Fig. 2)	I-state pH 7.8	1.08 ± 0.15	0.14 ± 0.02	4.22 ± 0.21	−2.29 ± 0.17
L50E (Fig. 2)	N*-state pH 4.0	0.97 ± 0.05	0.32 ± 0.01	3.47 ± 0.06	−2.43 ± 0.04
L50E (Suppl. Fig. 3, HX condition)	I-state pD_read 7.5	0.71 ± 0.02	0.18 ± 0.01	4.18 ± 0.05	−2.98 ± 0.07
L67E (Fig. 8)	I-state pH 7.8	0.63 ± 0.02	0.21 ± 0.01	4.31 ± 0.04	−4.00 ± 0.07
L67E (Fig. 8)	N*-state pH 4.0	0.68 ± 0.02	0.28 ± 0.01	4.10 ± 0.08	−4.39 ± 0.07