. Author manuscript; available in PMC: 2011 Apr 2.

Published in final edited form as: J Mol Biol. 2010 Feb 6;397(3):777–788. doi: 10.1016/j.jmb.2010.01.056

Table 2.

Native State Hydrogen Exchange on UbL50E intermediate, pD_read 7.5¹

2^nd structure of H-bond donor	H-bond donor (-NH)	H-bond acceptor (-C=O)	ΔG_HX (kcal mol⁻¹)	m (kcal mol⁻¹ M⁻¹)
β2 strand	I3	L15	2.90±0.05	1.03±0.09
	F4	S65	3.16±0.04	1.11±0.07
	V5	I13	3.10±0.01	1.12±0.03
	K6	L67	3.01±0.01	1.00±0.02
	T7	K11	3.20±0.09	1.16±0.16
β1 strand	I13	V5	3.06±0.03	1.04±0.06
	L15	I3	3.38±0.07	1.16±0.06
	V17	M1	3.48±0.03	1.16±0.06
α helix	V26	T22	3.21±0.06	1.00±0.15
	K27	I23	3.31±0.12	1.17±0.19
	A28	E24	3.55±0.04	1.18±0.07
	K29	N25	3.30±0.06	0.95±0.09
	Q31	K27	2.68±0.13	0.75±0.20
	E34	I30	2.71±0.03	0.77±0.05
β4 strand	R42	V70	2.87±0.04	0.93±0.08
	I44	N68	2.36±0.05	0.60±0.13
	W45	K48	~0¹	ND²
3₁₀ helix	L56	D21	1.71±0.11	0.47±0.29
	S57	P19	1.93±0.03	0.42±0.07
	D58	T55	1.86±0.03	0.32±0.07
	Y59	L56	1.73±0.01	0.23±0.02
β3 strand	L67	F4	2.14±0.16	0.73±0.25
	N68	I44	2.38±0.05	0.51±0.06
	L69	K6	2.51±0.03	0.57±0.08
	V70	R42	2.42±0.04	0.82±0.08
	R72	Q40	2.82±0.07	0.87±0.12

k_HX/k_int~ 1

Not determined.