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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biomaterials. 2010 Feb 24;31(14):3997–4008. doi: 10.1016/j.biomaterials.2010.01.144

Figure 4.

Figure 4

SDF-1α:stem cell interactions alter mast cell responses and inflammatory cell responses during the acute inflammatory response. Mast cell responses were monitored with Toluidine Blue staining. Control scaffolds have a more prominent mast cell response (A) with several degranulated mast cells present. In contrast, SDF-1α loaded scaffolds have far fewer mast cells with little degranulation (B). “S” indicates location of the scaffold implants The density of total and degranulated mast cells was quantified and revealed significant decrease in mast cell responses in SDF-1α scaffolds (C), Student t-test (P < 0.05). Inflammatory cell responses were monitored by examining CD11b+ expression. Control scaffolds have thick, dense bands of CD11b+ cells (red) (D) while SDF-1α scaffolds have a lesser inflammatory response in comparison (E). Arrow (↓) designates scaffold side of the interface. The density of CD11b+ cells was quantified at Days 3 & 7 and compared between treatment groups (F), revealing a substantial decrease in cell density in SDF-1α scaffolds after Day 3, Bonferonni test (P < 0.05).