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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biomaterials. 2010 Feb 24;31(14):3997–4008. doi: 10.1016/j.biomaterials.2010.01.144

Figure 5.

Figure 5

Localized SDF-1α release alters the tissue response to scaffold implants and influences cellular composition at the tissue interface. The histological appearance (H&E stain) of the cell response reveals a substantial accumulation of cells at the interface of control scaffolds (A), with a much lower density evident in SDF-1α treated scaffolds (B). The thickness of the cell layer (C) and the density of cells in the response region (D) were quantified at Day 7 and compared between groups, revealing significant decreases in the SDF-1α group for both parameters, Student t-test (P < 0.05). Based on the quantified cell density in H&E images, the population MSC (SSEA-4+ CD45−), HSC (c-kit+ cells), inflammatory cells (CD11b+), and fibroblasts (spindle shaped cells residing near the implant interface which do not stain positive for the aforementioned markers) were plotted, revealing substantial alteration in the cell composition between treatment groups (E), Student t-test between cell grouping (P < 0.05 as labeled with bracket and asterisk).