Fig. 5.

SMN binds more to dephosphorylated coilin in WI-38. a HeLa lysates were CIP- or mock-treated and immunoprecipitated with either antibodies against SMN or normal mouse serum (NMS). The reactions were subjected to SDS-PAGE and western transfer, followed by the detection of coilin and SMN with appropriate antibodies. Similar amounts of coilin were co-immunoprecipitated with anti-SMN antibody from CIP- and mock-treated HeLa lysates (lanes 3 and 4). No coilin or SMN were detected in the NMS control indicating that the CoIP experiment was specific for those proteins (lanes 5 and 6). b WI-38 lysates were CIP- or mock-treated followed by immunoprecipitation with anti-SMN antibodies and further processing as described above. Note that more coilin was co-immunoprecipitated from CIP-treated WI-38 lysate (lane 4) compared to that recovered using mock-treated lysate (lane 3). Input lanes represent 6% of the total protein used in the experiments