A: Representative current-clamp traces where mecamylamine (30 μM), a nAChR antagonist, was applied immediately after the end of levamisole application in the control (n = 4) and the test (n = 5) recordings. The first trace shows a control and the second trace shows the test response after AF2.
B: Bar graph comparing T80 controls and AF2 test pre-treatments in the presence of mecamylamine. AF2 potentiated the duration of levamisole secondary response T80, in the presence of mecamylamine (p < 0.05, unpaired t-test).