A: Representative current-clamp trace showing levamisole (1 μM) applications before and after AF2 treatment in the presence of 0.1 μM ryanodine.
B: Bar graph depicting T80 (min) in control and test applications. In the presence of 0.1 μM ryanodine, T80 in control and test responses were not significantly different (p > 0.05, n = 4, paired t-test). AF2 did not significantly potentiate the levamisole secondary response in the presence of ryanodine, as indicated by T80 measurements.