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. 2010 Mar 15;207(3):607–621. doi: 10.1084/jem.20091673

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© 2010 Rowland et al.

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Figure 2. — BCR-low B cells have impaired development in competition with normal B cells. (A) Schematic for the generation of mixed bone marrow chimeras. (B) Cells from mixed bone marrow chimeras described in A were analyzed by flow cytometry for the expression of IgM^a and IgM^b. Numbers represent the percentage of IgM^a (top, 3-83Igi; bottom, 3-83Igi-low) and IgM^b (CB17) cells within the total B220⁺IgM⁺ cell population in the bone marrow (left) and spleen (right). (C and D) Mean frequencies (±SD; n = 6–10 mice from two separate experiments) of 3-83Igi, CB17, and 3-83Igi-low donor cells in mixed 3-83Igi:CB17 (C) and 3-83Igi-low:CB17 (D) bone marrow chimeras. The following surface markers were used to discriminate B cell subsets: immature (bone marrow), IgM⁺IgD^–; transitional (bone marrow), IgM^highIgD^low; transitional (spleen), B220⁺CD24^high; and mature (spleen), B220⁺CD24^low. N.S., nonsignificant p-values of 0.41–0.43.