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. 2010 Mar 15;207(3):607–621. doi: 10.1084/jem.20091673

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© 2010 Rowland et al.

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Figure 3. — Lifespan of mature 3-83Igi-low B cells is normal in vitro and slightly reduced in vivo. (A) Spleen B cells from 3-83Igi and 3-83Igi-low mice were cultured with or without 20 ng/ml of recombinant BAFF. Viable cells were enumerated at the indicated times. Data represent arithmetic means ± SD from three mice analyzed over two independent experiments. (B) Scheme for the adoptive transfer of spleen B cells. (C) The frequency of 3-83Ig⁺ (B1-8H3-83κ–negative) donor B cells from adoptively transferred mice described in B was determined by flow cytometry before transfer (on day 0) and on days 21 and 41 after transfer. Shown are the arithmetic mean percentages (±SD; n = 4–10 pooled donor mice into three recipient mice) of 3-83Igi and 3-83Igi-low donor cells within the total CD19⁺ B cell population. Data are representative of two independent experiments.