Figure 1.

IL-12Rβ1 is required for M. tuberculosis–induced DC migration. (A) M. tuberculosis was instilled into the trachea of C57BL/6 mice, and 3 h later the frequency of CD11c⁺IL-12Rβ1⁺ cells in the lungs was determined. Dot plots are representative of four mice per condition; this experiment was performed twice with two separate BM preparations. (B) C57BL/6 BMDCs were exposed to M. tuberculosis or media alone, and 3 h later the frequency of CD11c⁺IL-12Rβ1⁺ cells was determined. Dot plots represent the same BMDC preparation stimulated with either condition and are representative of three separate experiments. (C) BMDCs generated from C57BL/6 or il12rb1^−/− mice were assayed for their ability to migrate to CCL19 in a transwell assay after a 3-h exposure to M. tuberculosis (CI, number moved in response to CCL19/number moved to media alone). Data points in C represent mean and SD of triplicate values and are representative of three separate experiments; for the difference between CI induced in C57BL/6 relative to il12rb1^−/− DCs, *, P < 0.05; **, P < 0.005, as determined by Student’s t test. (D–F) M. tuberculosis/CFSE was instilled via the trachea into C57BL/6, il12b^−/−, il12rb1^−/−, or il12rb2^−/− mice. 18 h later, the frequency (D and E) and total number of CD11c⁺CFSE⁺ cells in the draining MLN were counted. The data points in E and F represent the mean and SD of combined data from four mice per group (F) and are representative of two separate experiments; for the difference between percentage and/or number of CD11c⁺CFSE⁺ cells found in C57BL/6 mice relative to il12b^−/− or il12rb1^−/− mice, *, P < 0.05; ***, P < 0.0005, as determined by Student’s t test.