. Author manuscript; available in PMC: 2010 Nov 24.

Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):10882–10893. doi: 10.1021/bi901506f

Table 3.

Differences in free energies (ΔG°₃₇ in kcal/mol) resulting from substitution of U^M to s²U^M, U^M to s²U^L and U^L to s²U^L at particular positions within 2'-OMeRNA/RNA and LNA-2'-OMeRNA/RNA duplexes.

	5'-Terminal			3'-Terminal			Central			5'-Penultimate
	U^M→s²U^M (U^M→U^L)	U^M→s²U^L	U^L→s²U^L	U^M→s²U^M (U^M→U^L)	U^M→s²U^L	U^L→s²U^L	U^M→s²U^M (U^M→U^L)	U^M→s²U^L	U^L→s²U^L	U^M→s²U^M (U^M→U^L)	U^M→s²U^L	U^L→s²U^L
ΔΔG°₃₇ A-Y	−0.99 (−0.46)	−1.33	−0.87	0.17 (0.13)	−0.47	−0.60	−1.41 (−1.37)	−2.81	−1.44	−0.66 (−1.30)	−1.82	−0.52
ΔΔG°₃₇ G-Y	−0.24 (−0.22)	−0.66	−0.44	0.59 (−0.45)	−0.47	−0.02	−0.57 (−2.16)	−2.04	0.12	0.30 (−1.29)	−0.95	0.34
ΔΔΔG°₃₇ (A-Y) – (G-Y)	−0.25 (−0.24)	−0.67	−0.43	−0.42 (0.58)	0.00	−0.58	−0.84 (0.79)	−0.77	−1.56	−0.96 (−0.01)	−0.87	−0.86