Figure 3.

Peptide exchange occurs to the same extent in the presence or absence of exogenous β₂m. Purified K^b/Wt, K^b/PolyI, and K^b/PolyG complexes were incubated with the competitor peptides E6, K6, and K6, respectively (complex peptide/competitor; 1:200), in the presence (empty bars) or absence (filled bars) of exogenous β₂m (complex/β₂m; 1:1) in PBS at 37°C. Aliquots were withdrawn after 90-min incubation time, analyzed by IEF gel chromatography, and visualized by Coomassie staining. The amount of the newly formed K^b/peptide complex (K^b/K6, K^b/E6, and K^b/K6) at 90 min is indicated as percentage of exchange (%), as compared with their corresponding starting material (100%) of K^b/peptide complex (K^b/PolyI, K^b/Wt, and K^b/PolyG, respectively) at time zero.