Table 1.

Sequences, relative binding affinities, and isoelectric points of peptides used in this study

VSV-peptide analogs	Sequence	Relative binding affinity*	Isoelectric point (IP)
WtN52-59†	RGYVYQGL	Medium	10.3
K6	RGYVYKGL	Medium	10.7
E6	RGYVYEGL	Medium	8.2
PolyG	GGYGYGGL	Low	8.4
PolyI	IGYIYIGL	High	8.2

^*Relative binding affinity was assessed in competitive folding experiments of K^b H chain and β₂m together with a mixture of K6, E6, PolyG, and PolyI peptide against the Wt peptide (data not shown). The high relative binding affinity is approximately eight times higher than the medium; and the medium relative binding affinity is approximately 15 times higher than the low relative binding affinity. 

^†The K_D = 171 nmolar (0.17 μM) for the VSV Wt peptide at 37°C (35).