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. Author manuscript; available in PMC: 2010 Mar 16.
Published in final edited form as: J Mol Biol. 2008 Aug 9;383(1):122–132. doi: 10.1016/j.jmb.2008.08.008

Figure 5.

Figure 5

Measurement of homo- and heterotrimer solubility by gel electrophoresis. (a) Monomer and dimer gel bands of type I collagen chains in different initial mixtures. (b) Calibration curves for monomer (α1/α2) and dimer (α1α1/α1α2) fluorescent intensity ratios of the corresponding bands shown on the gel. (c) Concentrations of soluble homo-and heterotrimer in equilibrium with fibrils after overnight equilibration at 32 °C plotted vs. fibril composition. The soluble and fibrillar collagen fractions were separated by centrifugation and analyzed by gel electrophoresis.