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. 2009 Nov 10;18(1):135–142. doi: 10.1038/mt.2009.257

Figure 3.

Figure 3

Capsid-specific TCR cell line. (a) Jurkat/MA cells were transduced with a lentivirus expressing the α and β chains of the TCR specific for the AAV-capsid peptide VPQYGYLTL. Upon engagement of the TCR with the VPQYGYLTL peptide associated with HLA B*0702, the T cell becomes activated and expresses luciferase under control of the NFAT promoter that can be measured as relative light units on a standard luminometer. HHL5-B7 cells were either (b) loaded with VPQYGYLTL peptide for 4 hours or (c) transduced with AAV2 hAAT-hFIX for 24 hours before being washed and incubated with Jurma/VPQ reporter cells at an E:T ratio of 10:1. Cells were harvested after 24-hours incubation and assayed for luciferase expression. All RLU values are normalized by subtracting background Jurma/VPQ values. Experiments were performed in triplicate and (SD) is shown for each data point. AAV, adeno-associated virus; E:T, effector:target; hAAT, human α1-antitrypsin; hFIX, human Factor IX; NFAT, nuclear factor of activated T cells; RLU, relative light unit; TCR, T-cell receptor.