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. 2009 Nov 3;18(1):151–160. doi: 10.1038/mt.2009.251

Figure 6.

Figure 6

PCR and reverse transcriptase (RT)-PCR analysis on liver biopsies from nonhuman primates injected by intramuscular (Mac 14, 15, and 16) or by regional intravenous (Mac 19, 20, 21) with the AAV1-rtTA/Epo vector. Liver biopsies were performed between 15 and 18 months after rAAV injection. (a) Genomic DNA was extracted from liver biopsies and analyzed by PCR using primers specific for the rtTA sequence (269-bp amplicon) or for the endogenous GAPDH sequence as a positive control (219-bp amplicon). A liver biopsy obtained from a naive macaque was used as a negative control. A range of the pAAV-rtTA/Epo plasmid was used to determine the sensitivity of the rtTA-PCR reaction. (b) Total RNA was extracted from liver biopsies and analyzed by RT-PCR with (+RT) or without (−RT) reverse transcription using primers specific for the rtTA sequence (269-bp amplicon) or for the hypoxanthine guanine phosphoribosyltransferase (HPRT) sequence as a positive control (250-bp amplicon). A liver biopsy obtained from a naive macaque was used as a negative control, and 293 cells transfected with the pAAV-rtTA/Epo plasmid were used as positive controls (C+). A range of the pAAV-rtTA/Epo plasmid was used to determine the sensitivity of the rtTA-PCR reaction.