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. 2009 Sep 8;18(1):181–187. doi: 10.1038/mt.2009.207

Figure 2.

Figure 2

Evaluation of delivery for synthetic microRNA (miRNA) molecules to tumors in bone. A dual-luciferase expressing PC-3M cells that have 3'-UTR of Bcl2 under the renilla luciferase gene, PC-3M-luc/Rluc-Bcl2 3'UTR cells, were generated. These cells were used for dual assay system, for monitoring of tumor growth by firefly luciferase, for monitoring of delivery efficacy of synthetic miR-16 by renilla luciferase. (a) Representative images of bone metastasis in the femur of mice. To examine the efficacy of synthetic miR-16 in tumor cells, PC-3M-luc/Rluc-Bcl2 3'UTR cells were injected into the heart of nude mice. Nine weeks after tumor injection, bioluminescence from renilla luciferase was detected. Intravenous injection of miR-16 complexed with atelocollagen suppressed the expression of renilla luciferase (top). In contrast, bioluminescence from firefly luciferase was not affected (bottom). (b) Normalized fold change (2 days post/pre-miR-16 administration) of bioluminescence emitted from whole body of mice. This figure is graphically shown of the results of Figure 2a by fold change of photon counts. Data represent the mean (n = 3) ± SD *P < 0.01 versus NC miRNA. NS, not significant. NC, negative control.