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. 2009 Sep 15;18(1):188–197. doi: 10.1038/mt.2009.219

Figure 2.

Figure 2

JG-ODN delivery and activity. (a) Nondenaturing gel electrophoresis demonstrates the intracellular delivery of JG-ODNs. Lanes 1, 2, and 5: migration of free T40214, JG243, and JG244, respectively. Lanes 3 and 6: JG243/PEI and JG244/PEI in PANC1 cell lysates. Lanes 4 and 7: JG243 and JG244 without PEI in PANC1 cell lysates. (b) JG-ODNs decreased HIF-1α levels in PANC1 cancer cells. N, nonhypoxic conditions (lane 1). Hypoxic cells were exposed to ODN concentrations from 0 to 5.0 µmol/l (lanes 2–6). GQ-ODN T40214 is a Stat3 inhibitor and ns-ODN is a control nonspecific oligonucleotide. (c) JG243 and JG244 inhibited HIF-1α and HIF-2α activations in PC3 prostate cancer cells but did not inhibit the levels of p300 and (d) p-Stat3. HIF-1 hypoxia-inducible factor-1; IL-6, interleukin-6; ns ODN, nonspecific oligodeoxynucleotide; PEI, polyethylenimine.