Skip to main content
. 2010 Feb 8;159(5):1092–1105. doi: 10.1111/j.1476-5381.2009.00633.x

Figure 1.

Figure 1

Primary structure and cell surface expression of hGPR17. (A) Serpentine model of hGPR17. The highly conserved residues among rhodopsin-like 7TM receptors are indicated as black circles with white letters in each transmembrane helix and the conserved disulphide-bridge between the extracellular loop 2 and the conserved cysteine residue in TM3 denoted as a stapled line. The 28 amino acids comprising the longer N-terminus of the hGPR17-L splice variant are indicated as grey circles. (B) Expression of C-terminally eGFP-tagged hGPR17-L in transiently transfected HEK293 cells as detected by confocal microscopy. (C) Expression of C-terminally eGFP-tagged hGPR17-L in transiently transfected 1321N1 cells as detected by confocal microscopy. (D) Cell surface expression of FLAG-tagged hGPR17 isoforms in HEK293 cells transiently transfected with hGPR17-L, -S or pcDNA3 vector control as measured by ELISA. The results represent mean ± SEM of raw data from three independent experiments performed in quadruplicates. *P<0.05 by Student's t-test. 7TM, seven transmembrane; hGPR17-L, long GPR17 isoform.