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. 2010 Feb 8;159(5):1092–1105. doi: 10.1111/j.1476-5381.2009.00633.x

Figure 6.

Figure 6

Ticlopidine does not display inverse agonism or antagonism at hGPR17 isoforms. (A) Putative inverse agonism of Ticlopidine. HEK293 cells were transiently transfected with Gqi4myr, CREB-Luc reporter plasmid and receptor or pcDNA3 at 15 ng per well.and incubated with the ligand in varying concentrations. The results are corrected for background (pcDNA3-transfected cells) and given as % relative to the activity in absence of the ligand. The data represent mean ± SEM of two independent experiments performed in quadruplicates. (B) Putative antagonism of Ticlopidine. HEK293 cells were transfected as described in (A) and incubated with agonist (UDP or UDP-glucose) at 10 µM and Ticlopidine in varying concentrations. The results are presented as in (A). (C) hP2Y12 control. HEK293 cells were transfected as described in (A) and were incubated with the agonist MeSADP at 10 nM and Ticlopidine in varying concentrations. The results are presented as in (A). CREB, cAMP response element binding protein; hGPR17, GPR17 isoform.