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. 2010 Jan 29;159(5):1174–1186. doi: 10.1111/j.1476-5381.2009.00595.x

Figure 1.

Figure 1

mPGES-1 and COX-2 induction after glutamate exposure in cultures of rat hippocampal slices. (A) Western blot analysis for mPGES-1, COX-2, cPGES, mPGES-2 and COX-1 in the cultures of hippocampal slices 24 h after exposure to 1 mM glutamate (GLU) or vehicle (CTL) for 15 min. Representative data from three separate experiments are presented. (B) Summary data from immunoblotting with mPGES-1 antibody were scaled to a percentage of the control response (n = 3). (C and D) Time course of the expression of mPGES-1 and COX-2 mRNA after glutamate exposure. Quantitated data from real-time PCR analysis with the mPGES-1 (C) and COX-2 (D) primers were normalized to GAPDH (n = 3). **P < 0.01, *P < 0.05 versus the basal level (0 h). COX, cyclooxygenase; cPGES, mPGES, cytosolic/microsomal prostaglandin E synthase.