. Author manuscript; available in PMC: 2010 Aug 1.

Published in final edited form as: Nat Protoc. 2010 Feb 4;5(2):357–370. doi: 10.1038/nprot.2009.246

Table 1. Monoclonal antibodies used in Protocols A and B.

Lineage Cocktail 1 (Lin1) antibodies target CD3, CD14, CD16, CD19, CD20 and CD56

Protocol A - Monocyte subsets & B lymphocytes
Target antigen	Fluorochrome	Antibody clone	Antibody host species & isotype	Manufacturer	Product catalog number^*	Laser used	Bandpass filters (nm)	Long-pass dichroic mirror (nm)
CCR2	PE	48607.211	mouse IgG_2b	R&D Systems	FAB151P	blue	575/26	550
CD11b	APC	ICRF44	mouse IgG₁	BioLegend	301310	red	660/20
CD14^†	Pacific Orange	TüK4	mouse IgG_2a	Invitrogen	MHCD1430	violet	585/42	505
CD16^†	Pacific Blue	3G8	mouse IgG₁	BioLegend	302021	violet	450/50
CD19^†	Alexa Fluor 700	SJ25-C1	mouse IgG₁	Invitrogen	MHCD1929	red	730/45	710
CD62L	APC-Alexa Fluor 750	DREG-56	mouse IgG₁	Invitrogen	MHCD62L	red	780/60	755
CD83	APC	HB15e	mouse IgG₁	BD Biosciences	551073	red	660/20
CD86	PE	FUN-1	mouse IgG₁	BD Biosciences	555658	blue	575/26	550
CD209	PerCP-Cy5.5	DCN46	mouse IgG_2b	BD Biosciences	558263	red	695/40	685
CX₃CR1	FITC	2A9-1	rat IgG_2b	MBL International	D070-4	blue	530/30	505
HLA-DR	FITC	L243	mouse IgG_2a	BD Biosciences	307604	blue	530/30	505
HLA-DR^†	PerCP	L243	mouse IgG_2a	BD Biosciences	347364	red	695/40	685
BD Multicolor CD83/CD86/CD209	APC/PE/PerCP-Cy5.5	HB15e/FUN-1/DCN46	mouse IgG₁/IgG₁/IgG_2b	BD Biosciencs	334098	(see above)

Protocol B - DC subsets
Target antigen	Fluorochrome	Antibody clone	Antibody host species & isotype	Manufacturer	Product catalog number^*	Laser used	Bandpass filters (nm)	Long-pass dichroic mirror (nm)
CCR2	PE	(see above)
CD1c	PE	AD5-8E7	mouse IgG_2a	Miltenyi Biotec	130-090-508	blue	575/26	550
CD11b	Alexa Fluor 700	VIM12	mouse IgG₁	Invitrogen	CD11b29	red	730/45	710
CD11b	APC-Cy7	ICRF44	mouse IgG₁	BD Biosciences	557754	red	780/60	755
CD11c^†	APC	S-HCL-3	mouse IgG2b	BD Biosciences	333144	red	660/20
CD62L	APC-Cy7	DREG-56	mouse IgG₁	eBioscience	10-0629	red	780/60	755
CD101	PE	BB27	mouse IgG₁	Serotec	MCA2236PE	blue	575/26	550
CD123^†	pure^**	AC145	mouse IgG_2a	Miltenyi Biotec	130-090-940	violet (for Pacific Blue)	450/50
CD123^††	eFluor 450	6H6	mouse IgG₁	eBioscience	48-1239	violet	450/50
CD303	APC	AC144	mouse IgG₁	Miltenyi Biotec	130-090-905	red	660/20
HLA-DR^†	PerCP	(see above)
Lineage Cocktail 1 (Lin1)^†§	FITC	multiple	mouse IgG₁/IgG_2b	BD Biosciences	340546	blue	530/30	505

Catalog numbers can vary in the USA, the European Union and other countries.

^**

For conjugation with Pacific Blue using the Invitrogen Zenon Pacific Blue Mouse IgG_2a Labeling Kit.

^†

Anchor markers used in Protocols A and B.

^††

CD123–eFluor 450 can be a substitute for CD123–Pacific Blue.