. Author manuscript; available in PMC: 2010 Aug 1.

Published in final edited form as: Nat Protoc. 2010 Feb 4;5(2):357–370. doi: 10.1038/nprot.2009.246

Table 3. Physical properties and differential expression profile of surface phenotypes on CD16⁺, CD14⁺CD16⁺ and CD14⁺ monocytes (Mo), CD19⁺HLA-DR⁺ B cells, CD11c⁺ MDCs, CD123⁺ PDCs and CD16^hiCX3CR1⁻ neutrophils.

Parameters	CD16⁺ Mo	CD16⁺CD14⁺ Mo	CD14⁺⁺ Mo	CD19⁺HLA-DR⁺ B lymphocytes	CD11c⁺ MDC	CD123⁺ PDC	CD16^hi Neutrophils

FSC	low-int	low-int to high-int	high-int	low	low to int	low	high
SSC	int	int	int	low	int	low to int	high
HLA-DR	pos	pos	pos	pos	pos	pos	neg^*
CX₃CR1	high	int	low	neg	n.d.	neg^**	neg
CCR2	neg	int	high	neg	high	int	neg
CD11b	int	int	high	neg to int	low or neg	neg	pos
CD62L	low or neg	low	pos	int	high	high	pos
CD101	pos^†	pos^†	pos^†	pos^†	pos	neg	pos^†

FSC, forward scatter; SSC, side scatter.

Reported positive in specific diseases⁵².

^**

Reported negative by Geissmann et al.²⁰

^†

We (data not shown) and others⁵³ have demonstrated the surface expression of CD101 on these cells by flow cytometry.

Table 3. Physical properties and differential expression profile of surface phenotypes on CD16+, CD14+CD16+ and CD14+ monocytes (Mo), CD19+HLA-DR+ B cells, CD11c+ MDCs, CD123+ PDCs and CD16hiCX3CR1− neutrophils.