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. 2009 Oct 27;18(2):317–326. doi: 10.1038/mt.2009.249

Figure 5.

Figure 5

Long-term, stable correction of low-density lipoprotein receptor (LDLR) deficiency in clonal Chinese hamster ovary (CHO) a7 Ldlr−/− cells transfected with pEHZ-LDLR-LDLR. (a) DiI-LDL binding and internalization in clonal CHO a7 Ldlr−/− cells stably transfected with pEHZ-LDLR-LDLR showed functional complementation of LDL receptor function 80 generations post-transfection. Expression of LDLR was sensitive to incubation with sterols and statins. (b) Fluorescent imaging of clonal CHO a7 Ldlr−/− cells carrying pEHZ-LDLR-LDLR 240 generations post-transfection showed binding and internalization of DiI-LDL comparable to untransfected wild-type CHO cells. (c) DiI-LDL binding and internalization in clonal CHO a7 Ldlr−/− cells stably transfected with pEHZ-LDLR-LDLR showed functional complementation of LDLR function 240 generations post-transfection. Expression of LDLR was sensitive to incubation with sterols and statins. Means are from three independent experiments each repeated in quadruplicate. Results are mean ± SD.